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rmil22 210-22  (PeproTech)
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PeproTech rmil22 210-22
IL22 promotes intestinal adaptation and counteracts the dysbiosis seen after 75% SBR . ( A ) Body weight changes in WT mice treated with either PBS (n = 3 mice) or <t>rmIL22</t> (n = 4) after 75% SBR from PODs 1–7. ( B and C ) The length of villi in the ( B ) jejunum and the ( C ) ileum were measured under light microscopy. At least 30 well-oriented intact villi were counted per mouse. ( D and E ) Transcriptional levels of IL22-mediated antibacterial peptides ( D ) Reg3b and ( E ) Reg3g in the jejunum. ( F and G ) Transcriptional levels of ( F ) Reg3b and ( G ) Reg3g in the colon. ( H ) Transcriptional levels of Tnf-α in the jejunum were measured by quantitative PCR. ( I and J ) Relative abundance of ( I ) Proteobacteria and ( J ) and Bacteroidetes and Firmicutes were evaluated by quantitative PCR. Each dot represents a mouse. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001; and # P < .05.
Rmil22 210 22, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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IL22 promotes intestinal adaptation and counteracts the dysbiosis seen after 75% SBR . ( A ) Body weight changes in WT mice treated with either PBS (n = 3 mice) or rmIL22 (n = 4) after 75% SBR from PODs 1–7. ( B and C ) The length of villi in the ( B ) jejunum and the ( C ) ileum were measured under light microscopy. At least 30 well-oriented intact villi were counted per mouse. ( D and E ) Transcriptional levels of IL22-mediated antibacterial peptides ( D ) Reg3b and ( E ) Reg3g in the jejunum. ( F and G ) Transcriptional levels of ( F ) Reg3b and ( G ) Reg3g in the colon. ( H ) Transcriptional levels of Tnf-α in the jejunum were measured by quantitative PCR. ( I and J ) Relative abundance of ( I ) Proteobacteria and ( J ) and Bacteroidetes and Firmicutes were evaluated by quantitative PCR. Each dot represents a mouse. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001; and # P < .05.

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: A Central Role for Lipocalin-2 in the Adaptation to Short-Bowel Syndrome Through Down-Regulation of IL22 in Mice

doi: 10.1016/j.jcmgh.2020.04.006

Figure Lengend Snippet: IL22 promotes intestinal adaptation and counteracts the dysbiosis seen after 75% SBR . ( A ) Body weight changes in WT mice treated with either PBS (n = 3 mice) or rmIL22 (n = 4) after 75% SBR from PODs 1–7. ( B and C ) The length of villi in the ( B ) jejunum and the ( C ) ileum were measured under light microscopy. At least 30 well-oriented intact villi were counted per mouse. ( D and E ) Transcriptional levels of IL22-mediated antibacterial peptides ( D ) Reg3b and ( E ) Reg3g in the jejunum. ( F and G ) Transcriptional levels of ( F ) Reg3b and ( G ) Reg3g in the colon. ( H ) Transcriptional levels of Tnf-α in the jejunum were measured by quantitative PCR. ( I and J ) Relative abundance of ( I ) Proteobacteria and ( J ) and Bacteroidetes and Firmicutes were evaluated by quantitative PCR. Each dot represents a mouse. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001; and # P < .05.

Article Snippet: After a 75% SBR, WT mice were treated daily for 6 days via intraperitoneal injections with either 100 μL PBS as control or 100 μL PBS containing 4 μg rmIL22 (210-22; PeproTECH) as treatment.

Techniques: Light Microscopy, Real-time Polymerase Chain Reaction